IN-VITRO PHARMACOKINETIC INTERACTION STUDIES OF ESCITALOPRAM OXALATE
WHEN CO-ADMINISTERED WITH CAFFEINE/CAFFEINATED BEVERAGES
Krishna veni N., Gouthami B., Meyyanathan S.N. and Elango. K.
Department of Pharmaceutical Analysis, J.S.S. College of Pharmacy [A Constituent College of JSS
University, Mysore], Rocklands, Udhagamandalam, Tamilnadu-643001, India
Abstract:
The aim of the present study was to evaluate the influence of caffeine/caffeinated beverages on the in vitro dissolution kinetics of escitalopram oxalate as well as the usefulness of this type of invitro data to predict the modifications in invivo absorption processes as a consequence of different factors. Fitting of experimental data to different theoretical in vitro dissolution profiles was performed by non-linear regression methods and the statistical moments were calculated from raw experimental data. Water was used as the dissolution media. An RP HPLC method was used for the estimation of escitalopram oxalate. This method was carried out on a Hibar C18 (250 mm × 4.6 mm, id 5µ) using mobile phase containing 25mM potassium dihydrogen orthophosphate (pH adjusted to 3.0 with ortho phosphoric acid) and acetonitrile with the ratio of 60:40 with UV detection at 234 nm. The flow rate was maintained at 1.2 ml/min. The developed method was validated according to the ICH guidelines using various validation parameters. The dissolution study was carried out for one hour in the presence synthetic caffeine and green tea. The presence of synthetic caffeine and green tea decreases the release of escitalopram oxalate by 33.75 and 13.12% respectively.
